CRISPR/Cas technology’s endonuclease activity has spurred genome editing, bioanalytical sensors, and disease diagnostics, notably for SARS-CoV-2 variants. Multiplexing capabilities enable sensitive detection of nucleic acids, proteins, and more. Despite successes, challenges persist, like limited multiplexing assays and off-target effects. Microfluidic integration shows promise but complexity delays immediate application. Spectral limitations in fluorescence-based assays hinder biomolecule […]